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stemspam sfem ii media  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc stemspam sfem ii media
    Stemspam Sfem Ii Media, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemspam+sfem+ii+media/stemspam+sfem+ii+media/pmc10864002__mmc4-613-126-131
    Average 90 stars, based on 1 article reviews
    stemspam sfem ii media - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: PD-L1 expressing hematopoietic stem cells and uses
    Article Snippet: In Vitro Proliferation Assay and Glucose Challenge of Human CD34+ Cells— CD34+ cells were first isolated using magnetic beads (Milteny kit) from PBMCs obtained from blood samples of enrolled subjects. .. Next, CD34+ cells were stained with CFSE (FITC, Invitrogen C1157) and cultured for 72 hours at 37° C. in 5% CO2 in StemSpam SFEM II media (StemCell Technologies). ..

    Article Title: PD-L1 expressing hematopoietic stem cells and uses
    Article Snippet: In Vitro Proliferation Assay and Glucose Challenge of Human CD34+ Cells— CD34+ cells were first isolated using magnetic beads (Milteny kit) from PBMCs obtained from blood samples of enrolled subjects. .. Next, CD34+ cells were stained with CFSE (FITC, Invitrogen C1157) and cultured for 72 hours at 37° C. in 5% CO2 in StemSpam SFEM II media (StemCell Technologies). ..

    Cell Culture:

    Article Title: PD-L1 expressing hematopoietic stem cells and uses
    Article Snippet: In Vitro Proliferation Assay and Glucose Challenge of Human CD34+ Cells— CD34+ cells were first isolated using magnetic beads (Milteny kit) from PBMCs obtained from blood samples of enrolled subjects. .. Next, CD34+ cells were stained with CFSE (FITC, Invitrogen C1157) and cultured for 72 hours at 37° C. in 5% CO2 in StemSpam SFEM II media (StemCell Technologies). ..

    Article Title: PD-L1 expressing hematopoietic stem cells and uses
    Article Snippet: In Vitro Proliferation Assay and Glucose Challenge of Human CD34+ Cells— CD34+ cells were first isolated using magnetic beads (Milteny kit) from PBMCs obtained from blood samples of enrolled subjects. .. Next, CD34+ cells were stained with CFSE (FITC, Invitrogen C1157) and cultured for 72 hours at 37° C. in 5% CO2 in StemSpam SFEM II media (StemCell Technologies). ..

    Recombinant:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Modification:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Protease Inhibitor:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Selection:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Labeling:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Fluorescence:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Purification:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Reverse Transcription:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    SYBR Green Assay:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Cell Isolation:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER NF-kB (D14E12) Rabbit mAb Cell signaling Cat# 8242S, RRID: AB_10859369 STING (D2P2F) Rabbit mAb Cell signaling Cat# 13647S, RRID: AB_2732796 Anti-Mouse IFN-Beta (neutralizing antibody, Rabbit IgG) PBL assay science Cat# 32401-1, RRID: AB_10891517 Biological samples Human blood samples Sheba medical center, Tel Hashomer (Ramat Gan, Israel) 0226-13 Human blood samples Yale University School of Medicine (New Haven, CT, USA) 0609001869 Human blood samples Rambam Heath Care campus (Haifa, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Human blood samples Hadassah Medical Center (Jerusalem, Israel), Israel National Biobank for Research (Midgam) RMB-0631-17 Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium - high glucose Sigma Cat# D5796 Fetal Bovine Serum Biological Industries Cat# 10270-106 DPBS, no calcium, no magnesium Biological Industries Cat# 02-023-1A StemSpam SFEM II media StemCell Cat# 09605 1-methyl-3-nitro-1-nitrosoguanidine Apollo Scientific Cat# OR301388 Propidium Iodide Solution BioLegend Cat# 421301 STING inhibitor (H151) Cayman chemical Cat# 25857 Fix/Perm Buffer (4x) BioLegend Cat# 421401 Perm Buffer (10x) BioLegend Cat# 421402 Recombinant Mouse IFN-a (carrier-free) BioLegend Cat# 752802 Recombinant Murine IFN-g Peprotech Cat# 315-05 cOmplete Protease Inhibitor Cocktail Roche Cat# 11697498001 PhosSTOP Roche Cat# 4906845001 Critical commercial assays IFN alpha Mouse ELISA Kit Invitrogen Cat# BMS6027 Mouse Granzyme B DuoSet ELISA R&D Systems Cat# DY1865-05 LEGENDplex MU Th1/Th2 Panel (8-plex) w/ FP V03 BioLegend Cat# 741053 Proteome Profiler Mouse XL Cytokine Array R&D Systems Cat# ARY028 EasySep Mouse PE Positive Selection Kit II Stemcell Technologies Cat# 17666 Live Cell Labeling Kit - Red Fluorescence - Cytopainter abcam Cat# ab187965 Total RNA Purification Micro Kit Norgen Cat# 35300 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4374966 Fast SYBR Green Master Mix Bio-Rad Cat# 4385612 MojoSort Mouse CD8 T Cell Isolation Kit BioLegend Cat# 480008 MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit R&D Systems Cat# MAGM209 ELISA Mouse IL-6 BioLegend Cat# 431301 (Continued on next page) ll OPEN ACCESS Article e3 Cancer Cell 42, 253–265.e1–e12, February 12, 2024 ..

    Generated:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. Next, lentiviral particles were generated by co-transfecting HEK-293FT cells with packaging (psPAX2, Addgene Plasmid #12259) and envelope (pMD2.G, Addgene Plasmid #12259) plasmids together with pXPR_053 (control vector, Addgene Plasmid # 113591) or pXPR_053 vector containing gRNA specific for the Ly6E gene in StemSpam SFEM II media (Stemcell technologies, Cat# 09605). ..

    Plasmid Preparation:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. Next, lentiviral particles were generated by co-transfecting HEK-293FT cells with packaging (psPAX2, Addgene Plasmid #12259) and envelope (pMD2.G, Addgene Plasmid #12259) plasmids together with pXPR_053 (control vector, Addgene Plasmid # 113591) or pXPR_053 vector containing gRNA specific for the Ly6E gene in StemSpam SFEM II media (Stemcell technologies, Cat# 09605). ..

    Control:

    Article Title: Interferon-stimulated neutrophils as a predictor of immunotherapy response
    Article Snippet: .. Next, lentiviral particles were generated by co-transfecting HEK-293FT cells with packaging (psPAX2, Addgene Plasmid #12259) and envelope (pMD2.G, Addgene Plasmid #12259) plasmids together with pXPR_053 (control vector, Addgene Plasmid # 113591) or pXPR_053 vector containing gRNA specific for the Ly6E gene in StemSpam SFEM II media (Stemcell technologies, Cat# 09605). ..



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